Chitosanases: Cleavage specificity



The specificity of linkage recognition for various chitinolytic and chitosanolytic enzymes was usually investigated using 20-30%-acetylated chitosan as substrate. So far, the enzymes can be classified into five categories. Chitosanase classification into three distinct types was proposed by Fukamizo et al. (22)


Chitinases:
Bacterial chitinase
(family 18) (23)
Bacterial chitinase
(family 19)
Streptomyces griseus HUT 6037 (24)

Chitosanases:
Type I
Bacillus pumilus BN-262 (22)
Penicillium islandicum (25)
Streptomyces sp. N174 (26)
Type II
Bacillus sp. No 7-M (27)
Type III
Streptomyces griseus HUT 6037 (28)
Bacillus circulans MH-K1 (29)
Nocardia orientalis (30)
Bacillus circulans WL-12* (8)


* This enzyme was previously reported as b-1,3-1,4-glucanase (8).
(Linkage orientation: the future non-reducing end in oligosaccharide product is on right, and the future reducing end is on the left.)
(the symbols used in this chart were previously explained here)



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This page was created by Ryszard Brzezinski and Andrzej Neugebauer.
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Last updated: 1999/02/06